Most metals are designed for extreme conditions and are intended to be sterilized.
Gloves must be placed inside of an autoclavable biohazard bag and exposed to a steam setting gloves will melt slightly but will not burn when autoclaved in this manner.
Polystyrene (PS), polyvinyl chloride (PVC), nylon, acrylic, low-density polyethylene (LDPE), and high-density polyethylene (HDPE) lab ware and polyurethane tubing are not autoclavable under any conditions. Polycarbonate can also withstand high temperatures. Polypropylene containers are often used as secondary containers to hold materials that are autoclaved.
Polypropylene is an inexpensive resin that can resist autoclave temperatures.
When autoclaving liquids in Pyrex® containers, do not fill more than 2/3 full and do not seal the container.
Only Pyrex® or Type I borosilicate glass is autoclavable.
Any material contained in such a manner that it touches the interior surfaces of the autoclave.
Flammable, reactive, corrosive, toxic or radioactive materials.
Low density (LDPE) and High density polyethylene(HDPE).
Biohazard waste decontamination, in autoclave bags can be wet or dry.
Glassware that must be sterilized upright and/or can trap air.
Dry hard items, either unwrapped or in porous wrap.
Use with glass containers with vented closures 2/3 full only Dry material can be treated in a fast exhaust cycle, while liquids and biological waste require slow exhaust to prevent boiling over of super-heated liquids.
The rate of exhaust will depend upon the nature of the load.
Increased cycle time may be necessary depending upon the make-up and volume of the load.
To be effective, the autoclave must reach and maintain a temperature of 121° C for at least 30 minutes by using saturated steam under at least 15 psi of pressure.
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